The bone itself is actually a femur from a human skeleton.
- In Chapter 1: Moving Pictures, the bone can be found in the toolbox if opened near the Boris clone corpse found in the dissection room. Another toolbox with the bone inside is found in the room at the end of the basement.
- In Chapter 3: Rise and Fall, the bone is located at the start of the safe house, behind the suitcase box. The bone will not be obtainable until Henry opens the safe house's door with the lever. Giving it to Buddy Boris will result in him holding the bone in his mouth like an actual canine from throughout the chapter, but disappears from near the chapter's end from Accounting & Finance where Buddy Boris is shaking Henry gently to ensure if Henry's alright. Also, from one of the rooms from the first set of stairways located early on Level 9, there are several identical-looking bones located from the barrel, however there are unobtainable.
- In Chapter 4: Colossal Wonders, the bone can be created by using the Ink Maker on two occasions. However, it is unobtainable. Brute Boris will appear with a bone in his mouth, if Henry gave Buddy Boris a bone in the third chapter.
- In Chapter 5: The Last Reel, while the bone never reappeared physically, if Buddy Boris was given the bone in Chapter 3, Henry can use the Seeing Tool on Tom and a bone will appear in his mouth.
The bone returned in Boris and the Dark Survival as one of the items that Boris has to find in order to progress. There's also a hidden bone found in the lockers. Once obtained in the hub, Boris will chew on the bone throughout the whole game until the game restarts.
- Chapter 3: Rise and Fall
- Giving Buddy Boris the bone unlocks the "Knick-Knack Paddywhack" achievement.
- The description says "Got the poor doggie a bone.". However, Buddy Boris is technically a wolf, despite having close features of a dog.
- The achievement's name "Knick-Knack Paddywhack" along with its description is a reference to the children's 1906 nursery rhyme "This Old Man".
- After giving Buddy Boris a bone, if the player saves their data and restarts the game, the bone Buddy Boris is holding disappears.
- Chapter 5: The Last Reel
- If hacking back to where the Henry falls to the administration department, there is a bone lying on the ground. It is unknown if this is an Easter Egg or not.
|Objective items||Bendy Doll • Book • Door Lever • Dry Cell • End Reel • Extra-Thick Ink • Gear • Ink Heart • Inkwell • Wally's Keys • Pipe • Record • Special Gear • Turnwheel • Valve Core • Wrench|
|Non-objective items||Bacon Soup • Bone • Disc • Thick Ink|
|Weapons & Tools||Axe • Empty Bacon Soup Can • Flashlight • Gent Pipe • Pipe Wrench • Plunger • Scythe • Seeing Tool • Soup Bowl • Syringe • Tommy Gun|
|Supplies||Bone • Books • Gear • Music Radio • Plunger • Spoon • Wrench • Ink Heart|
|Collectibles||Scrap • Track • Tape • Odd Fuse • Lost Paper • Keys • Candle • Dance Blueprint|
|Misc.||Bacon Soup • Miner Hat • Record • Sammy's Mask|